《植物生理学报》 2014, 50(4): 401-406

ABC转运体位于H₂S上游参与盐胁迫诱导的拟南芥气孔关闭

吴延朋, 李洪旺, 侯丽霞, 张丹丹, 刘新*

青岛农业大学生命科学学院, 山东省高校植物生物技术重点实验室, 山东青岛266109

通信作者：刘新；E-mail: liuxin6080@126.com；Tel: 0532-88030311

摘　要：

本文以拟南芥野生型、ABC转运体缺失突变体(Atmrp4、Atmrp5和Atmrp4/5)为材料研究了硫化氢(hydrogen sulfide, H₂S)和ABC转运体在盐胁迫诱导拟南芥气孔关闭中的作用及其相互关系。结果表明, 盐胁迫能够引起拟南芥叶片AtMRP4及AtMRP5表达量显著升高, 诱导野生型拟南芥叶片气孔关闭, 但对Atmrp4、Atmrp5及Atmrp4/5气孔开度无显著影响; 而ABC转运体抑制剂格列本脲(glibenclamide, Gli)可减弱盐胁迫诱导的拟南芥气孔关闭的作用, 表明ABC转运体参与盐胁迫诱导的拟南芥气孔关闭过程。盐胁迫能够引起野生型拟南芥H₂S合成相关酶L-/D-半胱氨酸脱巯基酶(L-/D-CDes)活性及H₂S含量显著升高, 而ABC转运体抑制剂格列本脲处理后则没有这种变化, 同时盐胁迫也不能引起Atmrp4、Atmrp5及Atmrp4/5的L-/D-CDes活性及H₂S含量显著升高, 表明ABC转运体位于H₂S上游参与盐胁迫诱导气孔关闭过程。

关键词：硫化氢; ABC转运体; 盐胁迫; 气孔关闭

收稿：2013-11-08   修定：2014-01-13

资助：国家自然科学基金(31170237)和山东省自然科学基金(ZR2010CM024)。致谢 山东省泰山学者团队建设工程。

ATP-Binding Cassettee Transporter Signals Salt-Induced Stomatal Closure in Arabidopsis thaliana L. by H2S Pathway

WU Yan-Peng, LI Hong-Wang, HOU Li-Xia, ZHANG Dan-Dan, LIU Xin*

College of Life Sciences, Qingdao Agricultural University, Key Lab of Plant Biotechnology in Universities of Shandong Province, Qingdao, Shangdong 266109, China

Corresponding author: LIU Xin; E-mail: liuxin6080@126.com; Tel: 0532-88030311

Abstract:

Using Arabidopsis thaliana wild type, ATP-binding cassette (ABC) transporter deficient mutants Atmrp4, Atmrp5, Atmrp4/5 as materials, the participation of hydrogen sulfide (H2S) and ABC transporter signal pathway in salt-induced stomatal closure were studied. These results showed that AtMRP4 and AtMRP5 transcription rate in Arabidopsis leaves increased dramaticly under salt stress, and salt stress induced stomata closure in wild type, but had no effect on Atmrp4, Atmrp5 and Atmrp4/5. The ABC transporter inhibitor glibenclamide (Gli) inhibited the inducing effects of salt stress on stomata closure in wild type, it could be deduced that ABC transporter participate in salt-induced stomatal closure in Arabidopsis. Salt-induced increase in L-/ D-cysteine desulfhydrase (L-/D-CDes, enzymes participate in H2S production) activities and H2S content could be seen in wild type, but had no effect on wild type treated by Gli as well as Atmrp4, Atmrp5, Atmrp4/5. All these results indicated that ABC transporter functions upstream of H2S in salt-induced stomatal closure in Arabidopsis.

Key words: H2S; ABC transporter; salt stress; stomatal closure